mouse col2 antibody  (Developmental Studies Hybridoma Bank)

Journal: Animal Models and Experimental Medicine

Article Title: Chondrogenic commitment of human umbilical cord blood and umbilical cord‐derived mesenchymal stem cells induced by the supernatant of chondrocytes: A comparison study

doi: 10.1002/ame2.12515

Figure Lengend Snippet: The detection outcomes of genes and proteins associated with chondrogenesis ( n = 5/group). (A) Messenger RNA (mRNA) expression levels of the chondrocyte‐related genes ( Sox9 , Col2a1 , and Col1a1 ). (B) Detection of the chondrocyte‐related proteins (SOX9, COL2, and COL1) using Western blot (WB). (C) Immunofluorescence (IF) staining of the chondrocyte‐related protein (COL2), scale bars = 100 μm. (D) Quantitative analysis of WB results. (E) Quantification of IF results. a, human umbilical cord blood‐derived mesenchymal stem cells (hUCB‐MSCs) cultured alone; b, human umbilical cord‐derived mesenchymal stem cells (hUC‐MSCs) cultured alone; c, hUCB‐MSCs cultured with the conditioned medium of chondrocytes; d, hUC‐MSCs cultured with the conditioned medium of chondrocytes; ns, no significance; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: All samples were blocked using 5% bovine serum albumin (BSA) and subsequently incubated using mouse monoclonal antibodies against COL2 (R&D, USA) at a dilution of 1:100.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Derivative Assay, Cell Culture

Journal: European Cells and Materials

Article Title: Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large, load-bearingarticular cartilage defects

doi: 10.22203/ecm.v045a11

Figure Lengend Snippet: Fig. 7. Larger COL2 deposition from hMSCs on PCL-CHyA scaffolds compared with CHyA. COL2 immunohistochemical staining (1:100, SC-52658) on (a) CHyA and (b) PCL-CHyA scaffolds after 28 d in culture (scale bar = 1 mm).

Article Snippet: Sections were rinsed in PBS and incubated overnight at 4 °C with a mouse primary antibody for COL2 (SC52658, Santa Cruz) at a 1:100 dilution in 1 % IHC-BB.

Techniques: Immunohistochemical staining, Staining

Journal: Stem Cells International

Article Title: Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

doi: 10.1155/2019/6806540

Figure Lengend Snippet: Primers used in quantitative RT-PCR.

Article Snippet: After deparaffinization in xylene, the specimens were rehydrated and treated with 3% H 2 O 2 for 15 min. Then, the specimens were blocked with goat serum for 30 min at RT and incubated at 4°C overnight with a mouse monoclonal COL2 antibody (1 : 200, Abcam).

Techniques:

Journal: Stem Cells International

Article Title: Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

doi: 10.1155/2019/6806540

Figure Lengend Snippet: The differentiation of hiPSCs into NP-lCs. (a) Optical microscopy images of the differentiation process: hiPSCs (I and II), MCs (III and IV), and NP-lCs (V and VI). 4x (scale bar = 500 μ m), 10x (scale bar = 200 μ m). (c) Alcian blue staining and S-O staining of the differentiated NP-lCs. 4x (scale bar = 500 μ m), 10x (scale bar = 200 μ m). (d) qRT-PCR analysis of COL2, ACAN, CA12, and COL1 mRNA expression levels in NP-lCs, MCs, and hiPSCs; ∗∗ p < 0.001. (e) Immunofluorescence images of COL2 and ACAN staining in NP-lCs. 10x (scale bar = 100 μ m), 20x (scale bar = 50 μ m).

Article Snippet: After deparaffinization in xylene, the specimens were rehydrated and treated with 3% H 2 O 2 for 15 min. Then, the specimens were blocked with goat serum for 30 min at RT and incubated at 4°C overnight with a mouse monoclonal COL2 antibody (1 : 200, Abcam).

Techniques: Microscopy, Staining, Quantitative RT-PCR, Expressing, Immunofluorescence

Journal: Stem Cells International

Article Title: Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

doi: 10.1155/2019/6806540

Figure Lengend Snippet: Immunohistochemistry staining of COL2 in disc samples from different experimental groups. Scale bar = 1,000 μ m.

Article Snippet: After deparaffinization in xylene, the specimens were rehydrated and treated with 3% H 2 O 2 for 15 min. Then, the specimens were blocked with goat serum for 30 min at RT and incubated at 4°C overnight with a mouse monoclonal COL2 antibody (1 : 200, Abcam).

Techniques: Immunohistochemistry, Staining